Scientists have a need to know the composition, identity and quantity of the samples with which they are deal. Consequently, the Chromatography which is the separation of an analyze from a complicated mixture of similar constituents for qualitative or quantitative identification is a very important development technique of chemical and physical analysis. This course discuss one of most important methods of instrumental analysis in the different applications. There is no chemical industrial field has no need to gas chromatographic analysis. 

The quality of analysis depend more on the quality of the operator than that of the instrument, so this course will help laboratory staff to understand meaning and principals of gas chromatographic and their applications.

• The course is of interest for any person working in any analytical laboratory.
• Laboratory staff, Analyst, Chemists, Supervisors.

Unit 1

• Introduction
• History of Chromatography
• Type of Chemical Analysis
• Separation Analysis:
  • Separations in everyday life
  • Simple separation procedures
  • Classification of separation process
  • Chromatographic Separation
  • Types of Separation Chromatography
• Symbols and terms of Chromatography
• Physical forces and interactions chromatography
• Fundamentals of chromatography:
  • Definitions
  • Basic System Design
  • Concept of Polarity
  • Properties of Separation
  • Physiochemical Processes of Separation
  • Adsorption
  • Partition
  • Ion Exchange
  • Size Exclusion

Unit 2

• Type of Chromatographic Analysis
• Gas Chromatography:

• Objectives
• Definitions
• Introduction
• Principals
• Mobile and Stationary Phase
• Instrumentation of Gas Chromatography
  • Mobile Phase
  • Gas Supplies
  • Injection Ports and Valves:

• Packed Column Injectors
• Open Tubular Column Injectors
• Injection Methods and Techniques
  • Columns:
  • Open Tubular Capillary GC Column
  • Packed GC Column
  • Column Volume Characteristics
  • Selecting Stationary Phases
  • Configurations of the stationary phase

Unit 3

• Detectors

• Flame ionization Detector
• Thermal Conductivity Detector
• Other Detectors
  • Programmed Temperature GC
  • Gas Chromatography Analysis of Non-volatiles
  • Inorganic GC
  • Routine Maintenance
  • Evaluation of GC
  • Data Results:
  • Recorders & Data Acquisition
  • Data analysis
  • Data Acquisition and Processing
  • Data Processing
  • Application in drugs analysis
  • Safety Requirements

Unit 4

• Chemical laboratory measurement evolution
• Analytical Method validation
• Characteristic of Method validation:
• Statistical in analytical methods
• Specificity
• Linearity
• Range
• Accuracy&Precision
• Detection Limit
• Reputability, Reducibility
• Robustness
• Errors in Qualitative and Quantitative Analysis
• Correction of errors and improving accuracy
• Quality Assurance Program
• Quality Audit (Internal and External)
• Evaluation of analytical data
• Methods for uncertainty characterization
• Control Chart
• Study Performance and Reporting

Unit 5

• Application for Data Evaluation
• Practical exercises for method validation
• Practical exercises for Inter-Laboratory Performance test:
• F-test
• T-test
• Q-test
• Z-score
• Practical exercises for Uncertainty & Control Chart
• Manual Calculation for Uncertainty
• Used Excel Function for Uncertiainty
• Drawing Control Chart by Excel