Course details

Overview ?

Course Description:

Scientists have a need to know the composition, identity and quantity of the samples with which they are deal. Consequently, the Chromatography which is the separation of an analyze from a complicated mixture of similar constituents for qualitative or quantitative identification is a very important development technique of chemical and physical analysis. This course discuss one of most important methods of instrumental analysis in the different applications. There is no chemical industrial field has no need to gas chromatographic analysis.

Course Objectives:

The quality of analysis depend more on the quality of the operator than that of the instrument, so this course will help laboratory staff to understand meaning and principals of gas chromatographic and their applications.

Who should attend?

The course is of interest for any person working in any analytical laboratory.
Laboratory staff, Analyst, Chemists, Supervisors.

Course Outline:

Unit 1

Introduction
History of Chromatography
Type of Chemical Analysis
Separation Analysis:
- Separations in everyday life
- Simple separation procedures
- Classification of separation process
- Chromatographic Separation
- Types of Separation Chromatography
Symbols and terms of Chromatography
Physical forces and interactions chromatography
Fundamentals of chromatography:
- Definitions
- Basic System Design
- Concept of Polarity
- Properties of Separation
- Physiochemical Processes of Separation
- Adsorption
- Partition
- Ion Exchange
- Size Exclusion

Unit 2

Type of Chromatographic Analysis
Gas Chromatography:

Objectives
Definitions
Introduction
Principals
Mobile and Stationary Phase
Instrumentation of Gas Chromatography
- Mobile Phase
- Gas Supplies
- Injection Ports and Valves:

Packed Column Injectors
Open Tubular Column Injectors
Injection Methods and Techniques
- Columns:
- Open Tubular Capillary GC Column
- Packed GC Column
- Column Volume Characteristics
- Selecting Stationary Phases
- Configurations of the stationary phase

Unit 3

Detectors

Flame ionization Detector
Thermal Conductivity Detector
Other Detectors
- Programmed Temperature GC
- Gas Chromatography Analysis of Non-volatiles
- Inorganic GC
- Routine Maintenance
- Evaluation of GC
- Data Results:
- Recorders & Data Acquisition
- Data analysis
- Data Acquisition and Processing
- Data Processing
- Application in drugs analysis
- Safety Requirements

Unit 4

Chemical laboratory measurement evolution
Analytical Method validation
Characteristic of Method validation:
Statistical in analytical methods
Specificity
Linearity
Range
Accuracy&Precision
Detection Limit
Reputability, Reducibility
Robustness
Errors in Qualitative and Quantitative Analysis
Correction of errors and improving accuracy
Quality Assurance Program
Quality Audit (Internal and External)
Evaluation of analytical data
Methods for uncertainty characterization
Control Chart
Study Performance and Reporting

Unit 5

Application for Data Evaluation
Practical exercises for method validation
Practical exercises for Inter-Laboratory Performance test:
F-test
T-test
Q-test
Z-score
Practical exercises for Uncertainty & Control Chart
Manual Calculation for Uncertainty
Used Excel Function for Uncertiainty
Drawing Control Chart by Excel

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Day 2

Day 3

Day 4

Day 5

Training Methodology

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